Exploration of the HIF-1α/p300 interface using peptide and Adhiron phage display technologies

Exploration of the HIF-1α/p300 interface using peptide and Adhiron phage display technologies.

The HIF-1α/p300 protein-protein interaction plays a key role in tumor metabolism and thus represents a high value target for anticancer drug-development. Although several studies have identified inhibitor candidates using rationale design, more detailed understanding of the interaction and binding interface is necessary to inform development of superior inhibitors. In this work, we report a det...

High-affinity peptide-based collagen targeting using synthetic phage mimics: from phage display to dendrimer display.

Peptides derived from phage display typically show significantly weaker binding than their respective high affinity phage, which can bind to protein surfaces in a multivalent fashion. Here we show that mimicking key aspects of the multivalent architecture of the phage on an AB(5) dendritic wedge can enhance the affinity of a phage-display derived collagen binding peptide 100-fold (K(d) = 550 nM...

Automated Detection of Conformational Epitopes Using Phage Display Peptide Sequences

BACKGROUND Precise determination of conformational epitopes of neutralizing antibodies represents a key step in the rational design of novel vaccines. A powerful experimental method to gain insights on the physical chemical nature of conformational epitopes is the selection of linear peptides that bind with high affinities to a monoclonal antibody of interest by phage display technology. Howeve...

identification of new peptide ligands for epidermal growth factor receptor using phage display technology

identification of hitherto undefined b-cell epitopes by antibodies in the sera of vitiligo patients using phage-display peptide library

a random 12 mers phage library was used to screen a pool of immunoglo¬bulin fractions obtained from vitiligo patients. subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. this panel was tested using an elis a for their reactivity with pooled sera from patients and normal controls. among the 16 randomly selected clones, two of clones showed...